La persona a incorporar realizara tareas de técnico de laboratorio en el marco de los proyectos de Investigacion Traslacional en cancer de pulmón. Entre las tareas a desempeñar, se encuentran:
Organización del laboratorio, diseño y optimización de protocolos y gestión de pedidos
Experimentacion animal con ratones:
o Xenoinjertos, crecimiento de tumores y toma de biopsias y necropsias.
o Aplicación de fármacos por via subcutánea, sonda oral, via intravenosa y via traqueal
Extraccion de acidos nucleicos a partir de tumores en fresco, parafinados y congelados
Analisis de calidad y cantidad de RNA y DNA
PCR en tiempo real
Análisis de proteínas por western blot, ELISA, etc.
Cultivos celuares de líneas de raton y humanas de cancer de pulmón, y puntualmente de otros tipos tumorales. Amplificacion, puesta a punto de transfecciones, ensayos de proliferación y puesta a punto y determinación de IC50 de diversos fármacos en dichas lineas
REQUISITOS DE LOS SOLICITANTES:
Podrán solicitar esta plaza quienes reúnan los siguientes requisitos en el momento de finalizar el plazo de presentación de solicitudes:
Titulación:
a. Tecnico superior de laboratorio (FP)
Requisitos necesarios:
Experiencia en trabajo con ratones de laboratorio
Experiencia en técnicas básicas de biología molecular: qPCR, extracción de acidos nucleicos de biopsias: en fresco, congeladas o parafinadas, análisis de proteínas por western blot, ELISA, iTRAQ, uso del ChemiDoc, bioanalizador, transformaciones bacterianas, maxiprep, miniprep, geles de agarosa, etc.
Experiencia en cultivos celulares de líneas celulares y cultivos primarios: mantenimiento, amplificación, congelación y descongelación. Estudios de IC50 con fármacos.
Transfecciones celulares.
Experiencia de gestión de pedidos, estudio de presupuestos y organización del laboratorio
Experiencia en normalización y puesta a punto de los protocolos de laboratorio
Requisitos deseables:
" Se valoraran las publicaciones y/o comunicaciones a congresos
" Se valorara el conocimiento de herramientas informáticas
" Se valorara un conocimiento medio de ingles hablado y escrito
Enviar un email junto con la documentacion pertinente a:
arosado@cnio.es
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